With the rapid development of intelligent technology and unmanned underwater navigation technology, intelligent cooperative operation mode has brought new gains to underwater acoustic countermeasure and posed a new threat to the normal operation efficiency of acoustic epuipments.Aming at the urgent problem of underwater acoustic electronic defense, taking submarine as the research object, the cooperative operation mode of underwater manned and unmanned combat platform is studied, the gain of acoustic countermeasure under the background of intelligent operation is annalyzied, and suggestions to improve the acoustic electronic defense ability is put forward, which provides theoretical and methodologial support for effenctively seizing the underwater acoustic power in intelligent operation.